Chk1 (CHEK1) Rabbit Polyclonal Antibody

CAT#: TA374726

Phospho-Chk1-S345 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of C6 cells, using Phospho-Chk1-S345 pAb (TA374726) at 1:1000 dilution or Chk1 antibody (A7653).C6 cells were treated by ATP(5 mM) at 30℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit .|Exposure time: 5min.

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  Western blot analysis of extracts of various cell lines, using Phospho-Chk1-S345 antibody (TA374726).|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB,1:500 - 1:2000
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho S345
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic phosphorylated peptide around S345 of human Chk1 (NP_001265.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 43kDa/50kDa/54kDa
• Gene Name: checkpoint kinase 1
• Database Link:
  Entrez Gene 1111 Human
  UniProt ID O14757
• Background: The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.
• Synonyms: CHK1