B Raf (BRAF) Rabbit Polyclonal Antibody

CAT#: TA374058

Phospho-B-Raf-S445 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of Raji cells, using Phospho-B-Raf-S445 pAb (TA374058) at 1:2000 dilution or BRAF antibody (A0038).Raji cells were treated by PMA/TPA (200nM) for 30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 10s.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB,1:500 - 1:2000
• Reactivities: Human, Rat
• Modifications: Phospho S445
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic phosphorylated peptide around S445 of human BRAF (NP_004324.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 84kDa
• Gene Name: B-Raf proto-oncogene, serine/threonine kinase
• Database Link:
  Entrez Gene 673 Human
  UniProt ID P15056
• Background: This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene.
• Synonyms: B-Raf; B-RAF1; BRAF1; FLJ95109; MGC126806; MGC138284; p94; RAFB1