BAX Rabbit Polyclonal Antibody

CAT#: TA373931

Bax Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts from normal (control) and Bax knockout (KO) 293T cells, using Bax antibody (TA373931) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 5s.

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  Western blot analysis of extracts of various cell lines, using Bax antibody (TA373931) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 60s.

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  Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug Bax antibody (TA373931). Western blot was performed from the immunoprecipitate using Bax antibody (TA373931) at a dilition of 1:1000.

Specifications

Product Data

• Applications: IP, WB
• Recommended Dilution: WB,1:500 - 1:1000
  IF,1:50 - 1:100
  IP,1:50 - 1:200
• Reactivities: Human
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human Bax (NP_620116.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 4kDa/12kDa/15kDa/18kDa/19kDa/21kDa/24kDa
• Gene Name: BCL2 associated X protein
• Database Link:
  Entrez Gene 581 Human
  UniProt ID Q07812
• Background: The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.
• Synonyms: Bcl2-L-4; BCL2L4