COMT Rabbit Polyclonal Antibody

CAT#: TA370105

COMT rabbit polyclonal antibody

Product Images

Gel: 12%SDS-PAGE Lysate: 40 μg Lane 1-4: Hela 293T Jurkat and 231 cell lysates Primary antibody: TA370105 (COMT Antibody) at dilution 1/350 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds

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  Immunohistochemistry of paraffin-embedded Human lung cancer tissue using TA370105 (COMT Antibody) at dilution 1/25 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human lung cancer tissue using TA370105 (COMT Antibody) at dilution 1/25, treated with fusion protein. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: Hela，293T，Jurkat and 231 cell lysates
  IHC: 25-100
  Positive control: Human lung cancer
  Predicted cell location: Nucleus and Cytoplasm
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein of human COMT
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: 1 year
• Predicted Protein Size: 30 kDa
• Gene Name: catechol-O-methyltransferase
• Database Link:
  Entrez Gene 1312 Human
  UniProt ID P21964
• Background: Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters.
• Synonyms: catechol-O-methyltransferase; OTTHUMP00000197749; OTTHUMP00000197750