spindlin 1 (SPIN1) Rabbit Polyclonal Antibody

CAT#: TA368880

SPIN1 rabbit polyclonal antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane 1-2: Jurkat 293T cell lysates Primary antibody: TA368880 (SPIN1 Antibody) at dilution 1/600 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 30 seconds

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  Immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using TA368880 (SPIN1 Antibody) at dilution 1/50 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using TA368880 (SPIN1 Antibody) at dilution 1/50, treated with fusion protein. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA368880 (SPIN1 Antibody) at dilution 1/50 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA368880 (SPIN1 Antibody) at dilution 1/50, treated with fusion protein. (Original magnification: ×200)

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: Jurkat, 293T cell lysates
  IHC: 50-200
  Positive control: Human colorectal cancer
  Predicted cell location: Nucleus and Cytoplasm
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein of human SPIN1
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: 1 year
• Predicted Protein Size: 30 kDa
• Gene Name: spindlin 1
• Database Link:
  Entrez Gene 10927 Human
  UniProt ID Q9Y657
• Background: Chromatin reader that specifically recognizes and binds histone H3 both trimethylated at 'Lys-4' and asymmetrically dimethylated at 'Arg-8' (H3K4me3 and H3R8me2a) and acts as an activator of Wnt signaling pathway downstream of PRMT2. In case of cancer, promotes cell cancer proliferation via activation of the Wnt signaling pathway (PubMed:24589551). Overexpression induces metaphase arrest and chromosomal instability. Localizes to active rDNA loci and promotes the expression of rRNA genes (PubMed:21960006). May play a role in cell-cycle regulation during the transition from gamete to embryo. Involved in oocyte meiotic resumption, a process that takes place before ovulation to resume meiosis of oocytes blocked in prophase I: may act by regulating maternal transcripts to control meiotic resumption.
• Synonyms: OCR; SPIN; spindlin; SPNS1