MYL12B Rabbit Polyclonal Antibody

CAT#: TA367778

MYL12B rabbit polyclonal antibody

Product Images

Gel: 12%SDS-PAGE Lysate: 40 μg Lane: Human urinary bladder tissue lysate Primary antibody: TA367778 (MYL12B Antibody) at dilution 1/250 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 20 seconds

• 

  Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using TA367778 (MYL12B Antibody) at dilution 1/25 (Original magnification: ×200)

• 

  Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using TA367778 (MYL12B Antibody) at dilution 1/25, treated with synthetic peptide. (Original magnification: ×200)

• 

  Immunohistochemistry of paraffin-embedded Human lung cancer tissue using TA367778 (MYL12B Antibody) at dilution 1/25 (Original magnification: ×200)

• 

  Immunohistochemistry of paraffin-embedded Human lung cancer tissue using TA367778 (MYL12B Antibody) at dilution 1/25, treated with synthetic peptide. (Original magnification: ×200)

Show all 5 images

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: Human urinary bladder tissue lysate
  IHC: 30-150
  Positive control: Human cervical cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide of human MYL12B
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: 1 year
• Predicted Protein Size: 20 kDa
• Gene Name: myosin light chain 12B
• Database Link:
  Entrez Gene 103910 Human
  UniProt ID O14950
• Background: MYL12B is a regulatory subunit of myosin and plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. There are two groups of residues on the MYL12B that are phosphorylated by distinct kinases and have contrasting effects on myosin II biophysical properties.Phosphorylation at Thr18/Ser19 essentially “activates” the myosin molecule to produce force. The second group of phosphorylated residues is at the N-terminus of the MYL12B at Ser1, Ser2 and Thr9 and causes inhibitory effect on myosin activity.
• Synonyms: MLC-2; MLC-2A; MLC-B; MLC20; MRLC2; MYLC2B; OTTHUMP00000165808; SHUJUN-1