ULK1 Rabbit Polyclonal Antibody

CAT#: TA367743

ULK1 rabbit polyclonal antibody

Product Images

Gel: 6%SDS-PAGE Lysate: 40 μg Lane: Hela cell lysate Primary antibody: TA367743 (ULK1 Antibody) at dilution 1/250 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 20 seconds

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  Immunohistochemistry of paraffin-embedded Human breast cancer tissue using TA367743 (ULK1 Antibody) at dilution 1/25 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human breast cancer tissue using TA367743 (ULK1 Antibody) at dilution 1/25, treated with synthetic peptide. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 200-1000
  WB positive control: Hela cell lysate
  IHC: 20-100
  Positive control: Human breast cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide of human ULK1
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: 1 year
• Predicted Protein Size: 113 kDa
• Gene Name: unc-51 like autophagy activating kinase 1
• Database Link:
  Entrez Gene 8408 Human
  UniProt ID O75385
• Background: ULK1 and ULK2 (for UNC-51-like kinase) encode similar amino-terminal serine/threonine kinase domains, a proline/serine-rich (PS) domain, and a species conserved carboxyl-terminal domain. Both share homology with the UNC-51 kinase from Caenorhabditis elegans and the APG1 kinase in yeast, which are involved in axonal extension and growth, and autophagy, respectively. ULK1 maps to human chromosome 12q24.3 and is ubiquitously expressed . ULK2, also widely expressed, maps to mouse chromosome 11B1.3 and is expected to have a similar molecular weight as ULK1 in human. ULK1 and ULK2 are thought to auto-phosphorylate the PS domain in vitro, and the significant homology among vertebrates suggest that ULK1 and ULK2 are involved in the regulation of fundamental biological processes .
• Synonyms: ATG1; FLJ38455; FLJ46475; KIAA0722; UNC51; Unc51.1