ALKBH8 Rabbit Polyclonal Antibody

CAT#: TA365348

ALKBH8 rabbit polyclonal antibody

Product Images

Gel: 6%SDS-PAGE Lysate: 40 μg Lane 1-2: A549 and Jurkat cell lysates Primary antibody: TA365348 (ALKBH8 Antibody) at dilution 1/300 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 3 seconds

• 

  Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using TA365348 (ALKBH8 Antibody) at dilution 1/25 (Original magnification: ×200)

• 

  Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using TA365348 (ALKBH8 Antibody) at dilution 1/25, treated with fusion protein. (Original magnification: ×200)

• 

  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA365348 (ALKBH8 Antibody) at dilution 1/25 (Original magnification: ×200)

• 

  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA365348 (ALKBH8 Antibody) at dilution 1/25, treated with fusion protein. (Original magnification: ×200)

Show all 5 images

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: A549 and Jurkat cell lysates
  IHC: 25-100
  Positive control: Human cervical cancer
  Predicted cell location: Cytoplasm or Nucleus
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Full length fusion protein
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: 1 year
• Predicted Protein Size: 75 kDa
• Gene Name: alkB homolog 8, tRNA methyltransferase
• Database Link:
  Entrez Gene 91801 Human
  UniProt ID Q96BT7
• Background: ALKBH8 (alkylated DNA repair protein AlkB homolog 8) is a 664 amino acid protein that is encoded by a gene located on chromosome 11. ALKBH8 contains one RRM (RNA recognition motif) domain and belongs to the AlkB family of proteins. ALKBH8 is one of many homologs of the Escherichia coli protein AlkB. AlkB functions to protect DNA and RNA against damage from environmental methylating compounds by directly reversing 1-methyladenine (1-meA) and 3-methylcytosine (3-meC) cytotoxic alkylation lesions in DNA and RNA. The enzyme acts by oxidative demethylation, utilizing ferrous iron and alpha-ketoglutarate as cofactors, 2-oxoglutarate as a co-substrate and molecular oxygen as the oxidizing agent. Three isoforms exist for ALKBH8 due alternative splicing of the gene.
• Synonyms: ABH8; FLJ38204; MGC10235; OTTHUMP00000211286; OTTHUMP00000211287