BNP (NPPB) Rabbit Polyclonal Antibody

CAT#: TA364247

Rabbit polyclonal anti Prepro-BNP-32 (22-46) (hu); neat antiserum

Product Images

Typical titration curve of BNP-32 in a competitive ELISA with this antibody

Specifications

Product Data

• Applications: ELISA
• Recommended Dilution: This antibody has been tested and validated in ELISA against BNP-32 (22-46). Other applications like immunohistochemistry (IHC), FACS or Western Blot may work as well. Optimal dilutions should be determined by the end user.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide H-Ser-Pro-Lys-Thr-Met-Arg-Asp-Ser-Gly-Cys-Phe-Gly- Arg-Arg-Leu-Asp-Arg-Ile-Gly-Ser-Leu-Ser-Gly-Leu-Gly-Cys-Asn-Val- Leu-Arg-Arg-Tyr-OH, (Disulfide bond) coupled to carrier protein.
• Formulation: Neat undiluted antiserum, lyophilized, packaged under nitrogen. Reconstitute by adding 50µl distilled water. This will give the equivalent of undiluted antiserum.
• Concentration: N/A
• Conjugation: Unconjugated
• Storage: Original vial: at least one year at 4° - 8°C from date of delivery. Minimize repeated thawing and freezing of the antiserum by freezing aliquots at -20°C or below.
• Gene Name: natriuretic peptide B
• Database Link:
  Entrez Gene 4879 Human
  UniProt ID P16860
• Background: The natriuretic peptide family consists of three biologically active peptides: atrial natriuretic peptide (ANP), brain (or B-type) natriuretic peptide (BNP), and C-type natriuretic peptide (CNP). Among these, ANP and BNP are secreted by the heart and act as cardiac hormones. Both ANP and BNP preferentially bind to natriuretic peptide receptor-A (NPR-A) and exert similar effects. Expression and secretion of ANP and BNP are stimulated by various factors and are regulated via multiple signaling pathways. The human BNP precursor proBNP is proteolytically processed to BNP1-32 and N-terminal proBNP (NT-proBNP) within ventricular myocytes. Uncleaved proBNP as well as mature BNP1-32 and NT-proBNP is secreted from the heart, and its secretion is increased in patients with heart failure. This antibody was generated by immunization of rabbits with Prepro-BNP-32 (22-46) coupled to a carrier protein.
• Synonyms: BNP