Integrin beta 4 binding protein (EIF6) Rabbit Polyclonal Antibody

CAT#: TA357856

EIF6 Antibody - N-terminal region

Product Images

WB Suggested Anti-EIF6 Antibody Titration: 1.0 ug/ml Positive Control: Hela Whole CellEIF6 is supported by BioGPS gene expression data to be expressed in HeLa

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  Host: Rabbit Target Name: EIF6 Sample Type: Human 721_B Antibody Dilution: 1.0ug/mlEIF6 is supported by BioGPS gene expression data to be expressed in 721_B

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  Researcher: Rosa Carotenuto, Department of Structural and Functional Biology, University of Naples 'Federico II' Application: Western blotting Species + Tissue/Cell type: Xenopus laevis embryos How many ug's of tissue/cell lysate run on the gel: 1. 50 ug xenopus laevis embryos lysate (lysate buffer) 2. 50 ug xenopus laevis embryos lysate (HB buffer) Primary antibody dilution: 1:500 Secondary antibody: Goat Anti-Rabbit AP Secondary antibody dilution: 1:20,000

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  Rabbit Anti-EIF6 antibody Catalog Number: ARP61388 Formalin Fixed Paraffin Embedded Tissue: Human Kidney Primary antibody Concentration: 1:100 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20x Exposure Time: 0.5-2.0sec

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  Rabbit Anti-EIF6 antibody Catalog Number: ARP61388 Formalin Fixed Paraffin Embedded Tissue: Human Skin Primary antibody Concentration: 1:100 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20x Exposure Time: 0.5-2.0sec

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Specifications

Product Data

• Applications: WB
• Reactivities: Human, Frog
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: Expected reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Yeast, Zebrafish
  Homology: Cow: 86%; Dog: 93%; Guinea Pig: 93%; Horse: 93%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 86%; Yeast: 85%; Zebrafish: 93%
• Formulation: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
  Note that this product is shipped as lyophilized powder to China customers.
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 27kDa
• Gene Name: eukaryotic translation initiation factor 6
• Database Link:
  NP_002203
  Entrez Gene 3692 Human
  UniProt ID P56537
• Background: Hemidesmosomes are structures which link the basal lamina to the intermediate filament cytoskeleton. An important functional component of hemidesmosomes is the integrin beta-4 subunit (ITGB4), a protein containing two fibronectin type III domains. The protein encoded by this gene binds to the fibronectin type III domains of ITGB4 and may help link ITGB4 to the intermediate filament cytoskeleton. The encoded protein, which is insoluble and found both in the nucleus and in the cytoplasm, can function as a translation initiation factor and prevent the association of the 40S and 60S ribosomal subunits.
• Synonyms: b(2)gcn; CAB; eIF-6; EIF3A; gcn; ITGB4BP; p27(BBP); p27BBP

Reference Data

• Protein Families: Druggable Genome