Presenilin 1 (PSEN1) Rabbit Polyclonal Antibody

CAT#: TA357492

PSEN1 Antibody - middle region

Product Images

WB Suggested Anti-PSEN1 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Positive Control: Human brain

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  Lanes: 1. Mouse WT brain extract (80ug) 2. Rat brain extract (80ug) Primary Antibody Dilution: 2ug/ml Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience Secondary Antibody Dilution: 1: 20,000 Gene Name: PSEN1 Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science

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  Host: Rabbit Target Name: PSEN1 Sample Type: Human 721_B Antibody Dilution: 1.0ug/mlPSEN1 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells

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  Host: Rabbit Target Name: PSEN1 Sample Type: Human Adult Placenta Antibody Dilution: 1.0ug/ml

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  The PSEN1 antibody (#ARP58941) works fine in 4% paraformaldehyde fixed tissue sections (30 um), they were cut on a sliding, freezing microtome. We stained it overnight 1:500 followed with a biotinylated secondary, followed by an Avidin/peroxidase tertiary antibody, final staining with metal-intensified DAB. Similarly, both LRP antibodies (#ARP58562 &ARP58563) work fine (stain appropriately) with this procedure. We are still evaluating the BACE1 antibody, it seems to work, too. -Dr. Thomas van Groen

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  Immunohistochemistry with Small intestine tissue

• Amount and Sample Type: 500 ug mouse brain homogenate Amount of IP Antibody: 6 ug Primary Antibody: PSEN1 Primary Antibody Dilution: 1:500 Secondary Antibody: Goat anti-rabbit Alexa-Fluor 594 Secondary Antibody Dilution: 1:5000 Gene Name: PSEN1 Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science

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Specifications

Product Data

• Applications: IHC, IP, WB
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PSEN1
• Specificity: Expected reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
  Homology: Cow: 86%; Dog: 86%; Guinea Pig: 100%; Horse: 86%; Human: 100%; Mouse: 93%; Rabbit: 100%; Rat: 85%
• Formulation: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
  Note that this product is shipped as lyophilized powder to China customers.
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 53kDa
• Gene Name: presenilin 1
• Database Link:
  NP_000012
  Entrez Gene 19164 MouseEntrez Gene 5663 Human
  UniProt ID P49768
• Background: Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1; PSEN2) or in the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor, such that they either directly regulate gamma-secretase activity or themselves are protease enzymes. Several alternatively spliced transcript variants encoding different isoforms have been identified for this gene, the full-length nature of only some have been determined.
• Synonyms: AD3; FAD; OTTHUMP00000180589; PS-1; PS1; PSNL1; S182

Reference Data

• Protein Families: Druggable Genome, Protease, Transmembrane
• Protein Pathways: Alzheimer's disease, Neurotrophin signaling pathway, Notch signaling pathway, Wnt signaling pathway