TIGIT Mouse Monoclonal Antibody [Clone ID: 4A10]

CAT#: TA355122

Mouse Monoclonal Anti-TIGIT Antibody [4A10]

Product Images

Western blot analysis of TIGIT in over expressing HEK293 cells using RF16054 antibody at (A) 0.25 ug/ml, (B) 0.5 ug/ml, and (C) 1 ug/ml.

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  Immunohistochemistry of TIGIT in human stomach carcinoma tissue using TIGIT Antibody and control mouse IgG (corner box) at 2 ug/ml.

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  Immunocytochemistry of TIGIT in over expressing HEK293 cells using TIGIT antibody and control mouse IgG antibody (left corner box) at 1 ug/ml.

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  Immunofluorescence of TIGIT in over expressing HEK293 cells using TIGIT Antibody at 1 ug/ml.

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  Immunofluorescence of TIGIT in human stomach carcinoma tissue using TIGIT Antibody at 5 ug/ml.

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  Flow cytometry analysis of TIGIT over expressing HEK293 cells using TIGIT antibody at 1 ug/ml. Blue: untransfected HEK293 cells. Yellow: TIGIT over expressing HEK293 cells.

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Specifications

Product Data

• Clone Name: 4A10
• Applications: FC, IF, IHC, WB
• Recommended Dilution: IHC start at 2 ug/mL. IF start at 1 ug/mL. FC start at 1 ug/mL. ICC start at 1 ug/mL. WB start at 1 ug/mL.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: TIGIT antibody was raised against the extracellular domain of human TIGIT
• Formulation: TIGIT Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: TIGIT Antibody is supplied as protein A purified IgG1.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 26 kDa; Observed: 47 kDa
• Gene Name: T-cell immunoreceptor with Ig and ITIM domains
• Database Link:
  NP_776160
  Entrez Gene 201633 Human
  UniProt ID Q495A1
• Background: TIGIT Antibody: The T cell immunoreceptor with Ig and ITIM domains (TIGIT) is a member of the PVR (poliovirus receptor) family of immunoglobin proteins. It is expressed on several classes of T cells including follicular B helper T cells (TFH). TIGIT has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses .Similar to other immune checkpoint proteins such as PD-1, TIGIT is upregulated on exhausted T cells in chronic viral infections and cancer. Blockade of both TIGIT and PD-1 pathways leads to tumor rejection in mice suggesting that it may be of therapeutic use against cancer.
• Synonyms: DKFZp667A205; FLJ39873; VSIG9; VSTM3; WUCAM