GI24 (C10orf54) Mouse Monoclonal Antibody [Clone ID: 8E11]

CAT#: TA355119

Mouse Monoclonal Anti-VISTA Antibody [8E11]

Product Images

Titration curve analysis of VISTA antibody to detect recombinant VISTA in ELISA at decreasing concentrations.

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  Immunohistochemistry of VISTA in human lymphoma tissue with VISTA antibody at 2 ug/mL.

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  Immunofluorescence of VISTA in transfected HEK293 cells with VISTA antibody at 2 ug/mL.

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  Immunofluorescence of VISTA in human lymphoma tissue with VISTA antibody at 10 ug/mL.

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  Immunofluorescence of VISTA in human spleen tissue with VISTA antibody at 10 ug/mL.

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  Immunocytochemistry of VISTA in transfected HEK293 cells with VISTA antibody at 1 ug/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 ug/mL.

• Flow cytometry analysis of VISTA overexpressing HEK293 cells using VISTA antibody and control mouse IgG antibody at 10 ug/ml. Blue: Untransfected HEK293 cells. Yellow: VISTA overexpressing HEK293 cells.

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Specifications

Product Data

• Clone Name: 8E11
• Applications: ELISA, FC, IF, IHC
• Recommended Dilution: IHC start at 2 ug/mL. ICC start at 1 ug/mL. IF start at 2 ug/mL. FC start at 10 ug/mL.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: VISTA antibody was raised against the extracellular domain of human VISTA.
• Formulation: VISTA Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: VISTA Antibody is supplied as protein A purified IgG1.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 31 kDa; Observed: 47 kDa
• Gene Name: chromosome 10 open reading frame 54
• Database Link:
  NP_071436
  Entrez Gene 64115 Human
  UniProt ID Q9H7M9
• Background: VISTA Antibody: VISTA/B7-H5/platelet receptor Gi24 is a single-pass type I membrane protein located at the cell surface. It is an immunoregulatory receptor which can inhibit T-cell response and may promote differentiation of embryonic stem cells, by inhibiting the BMP4 signaling pathway. The protein can be cleaved by MMP14, and stimulate MMP14-mediated MMP2 activation.
• Synonyms: GI24; PP2135; SISP1