STAT3 Rabbit Polyclonal Antibody

CAT#: TA354956

Rabbit anti STAT3 (pY705) Polyclonal Antibody

Product Images

WB: The whole cell lysate derived from A431 with EGF stimulated (Lane 1) or without EGF stimulated (Lane 2) were immunoblotted by Rabbit anti-STAT3 (pY705) antibody at 1:500.

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  DB: 1 ug peptide was blot onto NC membrane A: STAT3 (pY705) (Phosphospecific) B: STAT3 (Paired Y705) (Non phosphospeicfic) were blotted at a 1:2000 dilution by: 1: Rabbit anti-STAT3 (Phospho specific), 2: Rabbit anti-STAT3 (Paired Y705)

Specifications

Product Data

• Applications: Dot, WB
• Recommended Dilution: WB 0.1-1 µg/ml ELISA N/A IP 2-5 µg/ml IHC 2-10 µg/ml FC Not tested
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide surrounding to the epitope -APYLK-- with phosphorylation sites at Tyr705 of STAT3 protein from human, mouse, rat, chicken dog and bovine origins.
• Formulation: This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 94 kDa
• Gene Name: signal transducer and activator of transcription 3
• Database Link:
  NP_003141
  Entrez Gene 20848 MouseEntrez Gene 6774 Human
  UniProt ID P40763
• Background: STAT proteins (Signal transducer and activator of transcription) belong to a family of cytoplasmic transcription factors that can be phosphorylated by a lignad binding to its cell surface receptor. The phosphorylation of STAT3 induces STAT dimerization, nuclear translocation and DNA binding. Stat3 is constitutively activated in a number of human tumors. Stat3-activated transcription seems to be regulated by serine phosphorylation at Ser727 and tyrosine phosphorylation at Tyr705.
• Synonyms: ADMIO; APRF; HIES

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Adipocytokine signaling pathway, Chemokine signaling pathway, Jak-STAT signaling pathway, Pancreatic cancer, Pathways in cancer