CDK1 Rabbit Polyclonal Antibody

CAT#: TA354670

Rabbit anti CDC2 Polyclonal Antibody

Product Images

WB: The A431 cell lysate resolved onto 12% SDS-PAGE, transferred onto NC membrane, and followed by an immunoblotting with Rabbit anti-CDC2 (CT) antibody (Lane 1) at 1:500, or with a preincubation of immunizing peptide (lane 2)

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  IHC: Human prostate tissue stained with Anti-CDC2 antibody, at 1:200 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
• Reactivities: Human, Chicken, Bovine
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to the C-terminus of CDC2 protein from human, rat and mouse origins
• Formulation: This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The Rabbit IgG is purified by Epitope Affinity Purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 38 kDa
• Gene Name: cyclin-dependent kinase 1
• Database Link:
  NP_001124301
  Entrez Gene 983 Human
  UniProt ID P06493
• Background: Cyclins and cyclin-dependent kinases (CDKs) control cell cycle progression by phosphorlating regulatory proteins. These cyclin-related proteins appear to affect cell structure and function independent of the cell cycle. Cyclins and CDKs have a role in the development and maintenance of cell- and tissue-restricted properties of differentiated cells. CDC2 (Cell Division Cycle 2), also known as CDK1 (Cyclin Dependent kinase 1), is a member of the CDK family of serine/threonin kinases. It is a highly conserved serine protein kinase that plays a key role in regulation of the cell cycle.The phosphorylation of CDC2 at Y15 and T14 during the G2 phase of the cell cycle inhibits CDC2 activity, while the dephosphorylation of Y15 and T14 by CDC25 phosphatase during the late G2 restores its activity.
• Synonyms: CDC28A; CDK1; DKFZp686L20222; MGC111195

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Stem cell - Pluripotency
• Protein Pathways: Cell cycle, Gap junction, Oocyte meiosis, p53 signaling pathway, Progesterone-mediated oocyte maturation