Actin (ACTA1) Rabbit Polyclonal Antibody

CAT#: TA354295

Rabbit anti Actin, skeletal muscle Polyclonal Antibody

Product Images

WB: Tissue lysate from Mouse skeletal muscle was resolved onto 12% SDS-PAGE and transferred onto NC Membrane, then probed by Anti-Actin antibody (skeletal muscle specific ; at 1:500 (Lane 2). An immunoreactive protein at ~42 kDa was observed.

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  IHC: Skeletal muscle tissue stained with Anti-Actin antibody, at 1:200 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
• Reactivities: Human, Rat, Rabbit, Guinea Pig
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide derived from N-terminus of human alpha skeletal muscle isoforms of actin. This sequence is identical in human, rat, mouse, dog, bovine, guinea pig, sheep and frog origins.
• Formulation: This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The Rabbit IgG is purified by Epitope Affinity Purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: ~42 kDa
• Gene Name: actin, alpha 1, skeletal muscle
• Database Link:
  NP_001091
  Entrez Gene 29437 RatEntrez Gene 58 Human
  UniProt ID P68133
• Background: The Actin protein, a component of the eukaryotic cytoskeleton, is highly conserved between multiple species. This ubiquitous protein involved in the formation of filaments that are a major component of the cytoskeleton. Interaction with myosin provides the basis of muscular contraction and many aspects of cell motility. The levels of the Actin protein may vary between different cell types, but are consistent within a given cell type, and for this reason, Actin protein levels are considered an internal control for protein loading.
• Synonyms: ACTA; ASMA; CFTD; CFTD1; CFTDM; MPFD; NEM1; NEM2; NEM3; SHPM

Reference Data

• Protein Families: Stem cell - Pluripotency