C3orf10 (BRK1) Rabbit Polyclonal Antibody

CAT#: TA351245

Rabbit Polyclonal Anti-BRK1 Antibody

Product Images

Gel: 10%SDS-PAGE Lysate: 40 μg Lane: A172 cells Primary antibody: TA351245 (BRK1 Antibody) at dilution 1/350 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 minute

• 

  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA351245 (BRK1 Antibody) at dilution 1/40 (Original magnification: ×200)

• 

  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA351245 (BRK1 Antibody) at dilution 1/40, treated with synthetic peptide. (Original magnification: ×200)

• 

  Immunohistochemistry of paraffin-embedded Human gastric cancer tissue using TA351245 (BRK1 Antibody) at dilution 1/40 (Original magnification: ×200)

• 

  Immunohistochemistry of paraffin-embedded Human gastric cancer tissue using TA351245 (BRK1 Antibody) at dilution 1/40, treated with synthetic peptide. (Original magnification: ×200)

Show all 5 images

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: A172 cells
  IHC: 50-200
  Positive control: Human thyroid cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide of human BRK1
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glyceroln
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 9 kDa
• Gene Name: BRICK1, SCAR/WAVE actin nucleating complex subunit
• Database Link:
  NP_060932
  Entrez Gene 101314 MouseEntrez Gene 55845 Human
  UniProt ID Q8WUW1
• Background: HSPC300 (hematopoietic stem cell protein 300) is also known as probable protein BRICK1 or C3orf10 (chromosome 3 open reading frame 10) and is a 75 amino acid protein that is expressed as two isoforms and localizes to both the cytoplasm and the cytoskeleton. HSPC300 is thought to regulate cytoskeletal organization and Actin polymerization. Free HSPC300 exists as homotrimers prior to its incorporation into the WAVE complex. The WAVE complex includes five proteins, one of which is HSPC300, that regulate the ARC (Arp2/3 complex) which is responsible for Actin nucleation and is Rac 1-dependent. Because HSPC300 is a highly conserved subunit of the WAVE complex across many species, it is thought to have the same or similar functions in many different organisms.
• Synonyms: C3orf10; hHBrk1; HSPC300; MDS027

Reference Data

• Protein Pathways: Regulation of actin cytoskeleton