MDM2 Rabbit Polyclonal Antibody

CAT#: TA350708

Rabbit Polyclonal Anti-MDM2 Antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane 1-2: Jurkat cells 231 cells Primary antibody: TA350708 (MDM2 Antibody) at dilution 1/200 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds

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  Immunohistochemistry of paraffin-embedded Human brain tissue using TA350708 (MDM2 Antibody) at dilution 1/40 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human brain tissue using TA350708 (MDM2 Antibody) at dilution 1/40, treated with synthetic peptide. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human prostate cancer tissue using TA350708 (MDM2 Antibody) at dilution 1/40 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human prostate cancer tissue using TA350708 (MDM2 Antibody) at dilution 1/40, treated with synthetic peptide. (Original magnification: ×200)

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 200-1000
  WB positive control: Jurkat and 231 cells
  IHC: 30-150
  Positive control: Human brain
  Predicted cell location: Nucleus or Cytoplasm
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide of human MDM2
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glyceroln
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 55 kDa
• Gene Name: MDM2 proto-oncogene
• Database Link:
  NP_002383
  Entrez Gene 4193 Human
  UniProt ID Q00987
• Background: This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.
• Synonyms: ACTFS; hdm2; HDMX

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Bladder cancer, Cell cycle, Chronic myeloid leukemia, Endocytosis, Glioma, Melanoma, p53 signaling pathway, Pathways in cancer, Prostate cancer, Ubiquitin mediated proteolysis