HES5 Rabbit Polyclonal Antibody

CAT#: TA349126

Rabbit Polyclonal Anti-HES5 Antibody

Product Images

Western blot analysis of HES5 in Raji cell lysate with HES5 antibody at 1 ug/mL (A) the absence and (B) the presence of blocking peptide.

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  Immunohistochemistry of HES5 in human spleen tissue with HES5 antibody at 5 ug/mL.

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  Immunofluorescence of HES5 in human spleen tissue with HES5 antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 - 2 ug/mL, IHC: 5 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: HES5 antibody was raised against a 19 amino acid peptide near the amino terminus of human HES5.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: HES5 antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 18 kDa; Observed: 17 kDa
• Gene Name: hes family bHLH transcription factor 5
• Database Link:
  NP_001010926
  Entrez Gene 15208 MouseEntrez Gene 79225 RatEntrez Gene 388585 Human
  UniProt ID Q5TA89
• Background: HES5 is a member of the HES family of transcriptional repressors, which are the mammalian homologs of the Drosophila Hairy and Enhancer of Split and contain basic Helix-loop-helix (bHLH) and Orange domains (1). The HES5 gene is a downstream target of the Notch signaling pathway and its expression is downregulated during human cartilage differentiation (2). HES5 can directly repress transcription of the E3 ligase FBW7-beta, creating a feedback loop that modulates Notch-mediated intestinal and neural stem cell fate decisions (3).
• Synonyms: bHLHb38

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS
• Protein Pathways: Notch signaling pathway