DEPTOR Rabbit Polyclonal Antibody

CAT#: TA349051

Rabbit Polyclonal Anti-DEPTOR Antibody

Product Images

Western blot analysis of DEPTOR in mouse liver tissue lysate with DEPTOR antibody at (A) 1 and (B) 2 ug/mL.

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  Immunohistochemistry of DEPTOR in mouse liver tissue with DEPTOR antibody at 5 ug/mL.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1 - 2 ug/mL, IHC: 5 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: DEPTOR antibody was raised against a 16 amino acid peptide near the center of human DEPTOR.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: DEPTOR antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 45 kDa; Observed: 42 kDa
• Gene Name: DEP domain containing MTOR-interacting protein
• Database Link:
  NP_073620
  Entrez Gene 64798 Human
  UniProt ID Q8TB45
• Background: DEPTOR is an mTOR (mammalian target of rapamycin)-interacting protein whose expression is negatively regualted by mTOR complex 1 (mTORC1) and mTORC2 (1). Loss of DEPTOR activates RP6SK1, Akt1, and SGK1, promotes cell growth and survival, and activates mTORC1 and mTORC2 kinase activities. DEPTOR expression is low in most cancers, but is often elevated in multiple myelomas (1). In respons to stimulation by mitogens, DEPTOR is rapidly phosphorylated leading to its ubiquitination and degradation, allowing mTOR to generate an auto-amplification loop to promotes its own full activation (2).
• Synonyms: DKFZp686F0270; MGC138150