PCNA Goat Polyclonal Antibody

CAT#: TA348967

Goat Polyclonal Anti-PCNA Antibody

Product Images

TA348967 (0.01 ug/ml) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  TA348967 (0.01 ug/ml) staining of NIH3T3 (A), Mouse Testis (B) and Rat Testis (C) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  TA348967 (0.03 ug/ml) staining of Pig Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 0.01-0.03 ug/ml
• Reactivities: Human, Mouse, Rat, Pig (Expected from sequence similarity: Dog, Cow)
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The immunogen for Anti-PCNA Antibody: Peptide with sequence C-NGNIKLSQTSNVD, from the internal region of the protein sequence according to NP_002583.1.
• Formulation: Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: lot specific
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: proliferating cell nuclear antigen
• Database Link:
  NP_002583
  Entrez Gene 18538 MouseEntrez Gene 25737 RatEntrez Gene 477166 DogEntrez Gene 5111 Human
  UniProt ID P12004
• Background: The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]
• Synonyms: ATLD2
• Note: Reported variants represent identical protein: NP_872590.1, NP_002583.1

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Base excision repair, Cell cycle, DNA replication, Mismatch repair, Nucleotide excision repair