KLF4 Rabbit Polyclonal Antibody

CAT#: TA337056

Rabbit Polyclonal KLF4 Antibody

Product Images

Western Blot: KLF4 Antibody TA337056 - Lysate from human 293 cells in the (1) absence and (2) presence of immunizing peptide, (3) mouse NIH 3T3, and (4) RAW cells probed with KLF4 antibody at 1 ug/ml (293) and 2 ug/ml (NIH 3T3 and RAW). Goat anti-rabbit IgG HRP secondary and PicoTect ECL substrate solution were used for this test.

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  Western Blot: KLF4 Antibody TA337056 - Total protein from Human PC3, Hek293 and Mouse Neuro2A cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-KLF4 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

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  Flow Cytometry: KLF4 Antibody TA337056 - Intracellular analysis using KLF4 antibody. Balb/c mouse splenocytes were probed using 1 ug of KLF4 antibody (red) and 1 ug of isotype control was used for this test, and an anti-rabbit IgG PE conjugated secondary .

Specifications

Product Data

• Applications: FC, ICC/IF, IHC, WB
• Recommended Dilution: Western Blot: 1-3 ug/ml, Immunohistochemistry-Paraffin: 10 ug/ml, Flow Cytometry: 1 ug/10^6 cells, Immunohistochemistry, Immunocytochemistry/ Immunofluorescence: 1:10-1:500
• Reactivities: Human, Mouse, Rat, Bovine, Primate
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A portion of amino acids 1-50 of human KLF4 was used as the immunogen for this antibody.
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at -20C. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Kruppel-like factor 4 (gut)
• Database Link:
  NP_004226
  Entrez Gene 16600 MouseEntrez Gene 114505 RatEntrez Gene 9314 Human
  UniProt ID O43474
• Synonyms: EZF; GKLF

Reference Data

• Protein Families: Adult stem cells, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors