RANK (TNFRSF11A) Mouse Monoclonal Antibody [Clone ID: 64C1385.1]

CAT#: TA337036

Mouse Monoclonal RANK/TNFRSF11A Antibody (64C1385.1)

Product Images

Dual RNAscope ISH-IHC: RANK/TNFRSF11A Antibody (64C1385.1) TA337036 - Formalin-fixed paraffin-embedded tissue sections of human liver were probed for RANK/TNFRSF11A mRNA (ACD RNAScope Probe, catalog # 412281; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using mouse anti-human (Novus Biologicals catalog # TA337036) at 5 ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). IHC signal is confined to plasma membrane.

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  Western Blot: RANK/TNFRSF11A Antibody (64C1385.1) TA337036 - Detection of RANK in RAW cells. Lane 1. detection of RANK in RAW, a mouse cell line. Lane 2 & 3. IP/analysis of RANK. RANK protein from RAW cell lysate was immunoprecipitated either with control antibody (lane 2) or this antibody. RANK was detected by western blot using this antibody.

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  Western Blot: RANK/TNFRSF11A Antibody (64C1385.1) TA337036 - Total protein from Raw 264.7 was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-RANK1 in 1% block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.

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  Western Blot: RANK/TNFRSF11A Antibody (64C1385.1) TA337036 - Total protein from MG63, human testis and mouse testis was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-RANK1 in 1% block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.

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  Flow Cytometry: RANK/TNFRSF11A Antibody (64C1385.1) TA337036 - Analysis on RAW cells using 5 ug of antibody. Shaded histogram represents cells without antibody; green represents isotype control; purple represents antibody. Image using the PE format of this antibody.

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  Flow Cytometry: RANK/TNFRSF11A Antibody (64C1385.1) TA337036 - Analysis of antibody on (a) untreated BALB/c mouse splenocytes and (b) BALB/c mouse splenocytes stimulated with 5 ug/ml ConA for 72 hours. Cells were stained with 2 ug of antibody per 1x10^6 cells. Green represents isotype control; red represents antibody. Image using the PE format of this antibody.

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Specifications

Product Data

• Clone Name: 64C1385.1
• Applications: CyTOF-ready, FC, IHC, IP, WB
• Recommended Dilution: Immunohistochemistry, Flow Cytometry: 5 ug/10^6 cells, Western Blot: 1-2 ug/ml, Immunoprecipitation: 1-2 ug/ml, Dual RNAscope ISH-IHC, CyTOF-ready, Immunohistochemistry-Paraffin: 5 ug/ml
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1, lambda
• Clonality: Monoclonal
• Immunogen: A recombinant protein containing the extracellular domain (amino acids 33-208) of human RANK was used as immunogen.
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: tumor necrosis factor receptor superfamily member 11a
• Database Link:
  NP_003830
  Entrez Gene 21934 MouseEntrez Gene 8792 Human
  UniProt ID Q9Y6Q6
• Synonyms: CD265; FEO; LOH18CR1; ODFR; OFE; OPTB7; OSTS; PDB2; RANK; TRANCER

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction