CD105 (ENG) Mouse Monoclonal Antibody [Clone ID: 3A9]

CAT#: TA336920

Mouse Monoclonal Endoglin/CD105 Antibody (3A9)

Product Images

ELISA: Endoglin/CD105 Antibody (3A9) TA336920 - Red: Control Antigen (100 ng), Purple: Antigen (10 ng), Green: Antigen (50 ng), Blue: Antigen (100 ng).

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  Western Blot: Endoglin/CD105 Antibody (3A9) TA336920 - Analysis using CD105 mAb against HEK293 (1) and CD105 (AA: 331-567)-hIgGFc transfected HEK293 (2) cell lysates.

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  Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody (3A9) TA336920 - Analysis of paraffin-embedded kidney cancer tissues (left) and stomach cancer tissues (right) using CD105 mouse mAb with DAB staining.

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  Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (3A9) TA336920 - Analysis of HepG2 cells using CD105 mouse mAb (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red).

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  Western Blot: Endoglin/CD105 Antibody (3A9) TA336920 - Whole cell protein from HUVEC was separated on a 7.5% gel polyacrylamide gel and transferred to PVDF membrane. The membrane was probed with anti-CD105 antibody at 2 ug/ml and detected with an anti-mouse HRP labeled secondary antibody using chemiluminescence.

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Specifications

Product Data

• Clone Name: 3A9
• Applications: ELISA, ICC/IF, IHC, WB
• Recommended Dilution: Immunocytochemistry/ Immunofluorescence: 1:200-1:1000, ELISA: 1:10000, Western Blot: 1:500-1:2000, Immunohistochemistry-Paraffin: 1:200-1:1000, Immunohistochemistry
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human CD105 expressed in E. coli. [UniProt# P17813]
• Formulation: PBS, 0.03% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Ammonium sulfate precipitation
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 71 kDa
• Gene Name: endoglin
• Database Link:
  NP_000109
  Entrez Gene 2022 Human
  UniProt ID P17813
• Background: This gene encodes a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds TGFB1 and TGFB3 with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia.
• Synonyms: END; HHT1; ORW1
• Note: This CD105 (3A9) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence, Flow Cytometry and ELISA.

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane