Dynamin 1 (DNM1) Mouse Monoclonal Antibody [Clone ID: 3G4B6]

CAT#: TA336537

Mouse Monoclonal Dynamin Antibody (3G4B6)

Product Images

Simple Western: Dynamin Antibody (3G4B6) TA336537 - Simple Western lane view shows a specific band for Dynamin in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

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  Immunohistochemistry-Paraffin: Dynamin Antibody (3G4B6) TA336537 - Immunohistochemical analysis of paraffin-embedded human Lymph tissue, glioma tissue and cerebellum tissue, showing membrane localization using Dynamin antibody with DAB staining.

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  Flow Cytometry: Dynamin Antibody (3G4B6) TA336537 - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Dynamin antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.

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  Western Blot: Dynamin Antibody (3G4B6) TA336537 - Western blot analysis using anti-Dynamin monoclonal antibody against truncated Dynamin recombinant protein (1), SK-N-SH (2) and NIH3T3 (3) cell lysate.

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  Western Blot: Dynamin Antibody (3G4B6) TA336537 - Western blot analysis using Dynamin mouse mAb against C6 (1), NIH/3T3 (2), SKN-SH (3), LN18 (4), SHSY5Y (5) cell lysate and rat brain tiisues lysate (6).

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Specifications

Product Data

• Clone Name: 3G4B6
• Applications: ELISA, FC, IHC, IP, Simple Western, WB
• Recommended Dilution: Immunohistochemistry-Paraffin: 1:200- 1:1000, Immunohistochemistry-Frozen: 1:200- 1:1000, ELISA: 1:10000, Immunohistochemistry: 1:10-1:500, Simple Western: 1:50, Flow Cytometry: 1 ug per million cells, Western Blot: 1:500- 1:2000, Knockout Validated, Immunoprecipitation
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human Dynamin expressed in E. coli. [UniProt# Q05193]
• Formulation: Preservative: 0.05% Sodium Azide. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Ascites
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 97 kDa
• Gene Name: dynamin 1
• Database Link:
  NP_004399
  Entrez Gene 1759 Human
  UniProt ID Q05193
• Background: Dynamin (Dynamin-1, Dyn1), with 864-amino acid protein (about 95kDa), belongs to the dynamin family. Dynamin (neuron-specific), Dynamin-2 (ubiquitously expressed), and Dynamin-3 (expressed only in the testis, brain, and lung), constitute the dynamin family. Members of the dynamin family are GPTase, microtubule-associated proteins which are involved in endocytosis, synaptic transmission and neurogenesis. Dynamin is phosphorylated in nerve terminals exclusively in the cytosolic compartment and in vitro by protein kinase C. Dynamin is a large GTPase enzyme required in membrane constriction and fission during multiple forms of endocytosis. Dynamin is also a key molecule required for the recycling of synaptic vesicles in neurons, and it has been known that Dynamin gene expression is induced during neuronal differentiation.
• Synonyms: DNM
• Note: This Dynamin (3G4B6) antibody is useful for Western Blot, Immunohistochemistry on paraffin-embedded sections and ELISA.

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, Fc gamma R-mediated phagocytosis