TLR7 Rabbit Polyclonal Antibody

CAT#: TA336419

Rabbit Polyclonal TLR7 Antibody

Product Images

Flow (Intracellular): TLR7 Antibody TA336419 - Analysis using the FITC conjugate of TA336419. Staining of TLR7 in TLR7/HEK 293 cells using this antibody at 2 ug/10^6 cells. Green: Vector/HEK 293 cells. Red: TLR7/HEK 293 cells.

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  Flow Cytometry: TLR7 Antibody TA336419 - Analysis using the PE conjugate of TA336419. Staining of TLR7 in human PBMCs (monocytes) using 0.5 ug/10^6 cells of NBP2-24761. The shaded histogram represents cells alone. Green: PE-conjugated rabbit IgG isotype control (20304D). Red: TLR7 antibody.

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  Flow Cytometry: TLR7 Antibody TA336419 - Intracellular flow analysis of TLR7 in 10^6 ThP1 cells using 2 ug of TA336419. Shaded histogram represents cells without antibody. Green: isotype control. Red: TLR7 antibody. Intracellular flow kit was used for this test, and an anti-rabbit IgG PE conjugated secondary.

Specifications

Product Data

• Applications: FC, IHC
• Recommended Dilution: Flow Cytometry, Flow (Intracellular): 0.5 - 2 ug/1x10^6 cells, Immunohistochemistry: 1:10 - 1:500, Immunohistochemistry-Paraffin: 1:10 - 1:50, Flow (Cell Surface), Immunohistochemistry-Frozen: 1:20
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: This antibody was developed against KLH-conjugated synthetic peptide corresponding to a portion of amino acids 684-701 of human TLR7 (NP_057646).
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: toll like receptor 7
• Database Link:
  NP_057646
  Entrez Gene 170743 MouseEntrez Gene 51284 Human
  UniProt ID Q9NYK1
• Background: The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described. Stimulation of the NFkB signaling pathway by TLR7 suggests that it plays a role in immune response.
• Synonyms: TLR7-like
• Note: Flow Cytometry: (Cell Surface): see Wong et al, 2007 for details Immunohistochemistry-Frozen: (1:20) (see product citation, Chen et al., 2005)

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Toll-like receptor signaling pathway