KDM1A Mouse Monoclonal Antibody [Clone ID: 1B2E5]

CAT#: TA336270

Mouse Monoclonal LSD1 Antibody (1B2E5)

Product Images

Simple Western: LSD1 Antibody (1B2E5) TA336270 - Simple Western lane view shows a specific band for LSD1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.

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  Immunohistochemistry-Paraffin: LSD1 Antibody (1B2E5) TA336270 - Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma tissue, showing nuclear localization using LSD1 antibody with DAB staining.

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  Immunohistochemistry-Paraffin: LSD1 Antibody (1B2E5) TA336270 - Immunohistochemical analysis of paraffin-embedded Human Kidney Carcinoma tissue, showing nuclear localization using LSD1 antibody with DAB staining.

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  Western Blot: LSD1 Antibody (1B2E5) TA336270 - Analysis of LSD1 expression in Hela (1) and Jurkat (2) whole cell lysates.

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  Western Blot: LSD1 Antibody (1B2E5) TA336270 - Western blot analysis using LSD1 mouse mAb against COS (1), Hela (2), NIH/3T3 (3), A549 (4) and Jurkat (5) cell lysate.

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Specifications

Product Data

• Clone Name: 1B2E5
• Applications: ChIP, ELISA, IHC, Simple Western, WB
• Recommended Dilution: Chromatin Immunoprecipitation (ChIP), ELISA: 1:10000, Immunohistochemistry: 1:200-1:1000, Western Blot: 1:500-1:2000, Immunohistochemistry-Paraffin: 1:200-1:1000, Simple Western: 1:500, Knockdown Validated
• Reactivities: Human, Mouse, Primate
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human LSD1 (between amino acids 400-600) expressed in E. coli. [UniProt# O60341]
• Formulation: Preservative: 0.05% Sodium Azide. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Ascites
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 93 kDa
• Gene Name: lysine demethylase 1A
• Database Link:
  NP_001009999
  Entrez Gene 99982 MouseEntrez Gene 23028 Human
  UniProt ID O60341
• Background: The amine oxidase domain 2 AOF2 gene encodes a nuclear protein (LSD1,~95 kDa) containing a Swirm domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. LSD1 is a chromatin-modifying enzyme, which serves as a docking module for the stabilization of the associated corepressor complex(es) on chromatin.
• Synonyms: AOF2; BHC110; CPRF; KDM1; LSD1
• Note: This LSD1 (1B2E5) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections and ELISA.

Reference Data

• Protein Families: Druggable Genome, Transcription Factors