PPM1D Rabbit Polyclonal Antibody

CAT#: TA332984

Rabbit anti-PPM1D Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using PPM1D antibody.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB 1:500 - 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human PPM1D
• Formulation: Store at -20°C (regular) and -80°C (long term). Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 605
• Gene Name: protein phosphatase, Mg2+/Mn2+ dependent 1D
• Database Link:
  NP_003611
  Entrez Gene 53892 MouseEntrez Gene 287585 RatEntrez Gene 8493 Human
  UniProt ID O15297
• Background: The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The expression of this gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase, MAPK/p38, through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. This gene is located in a chromosomal region known to be amplified in breast cancer. The amplification of this gene has been detected in both breast cancer cell line and primary breast tumors, which suggests a role of this gene in cancer development.
• Synonyms: PP2C-DELTA; WIP1

Reference Data

• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: p53 signaling pathway