hnRNP K (HNRNPK) Rabbit Polyclonal Antibody

CAT#: TA332421

Rabbit anti-HNRNPK Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using HNRNPK antibody.

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  Immunofluorescence analysis of MCF7 cell using HNRNPK antibody. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of A549 cell using HNRNPK antibody.

Specifications

Product Data

• Applications: ICC/IF, IHC, WB
• Recommended Dilution: WB 1:500 - 1:2000;IF 1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human HNRNPK
• Formulation: Store at -20°C (regular) and -80°C (long term). Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 51 kDa
• Gene Name: heterogeneous nuclear ribonucleoprotein K
• Database Link:
  NP_002131
  Entrez Gene 15387 MouseEntrez Gene 117282 RatEntrez Gene 3190 Human
  UniProt ID P61978
• Background: Heterogeneous nuclear ribonucleoprotein K (hnRNP K) belongs to a family of RNA binding multiprotein complexes (hnRNP proteins) that facilitate pre-mRNA processing and transport of mRNA from the nucleus to cytoplasm (1-3). hnRNP K contains three unique structural motifs termed KH domains that bind poly(C) DNA and RNA sequences (4,5). Intricate architecture enables hnRNP K to facilitate mRNA biosynthesis (6), transcriptional regulation (7), and signal transduction. Research studies have shown that cytoplasmic hnRNP K expression is increased in oral squamous cell carcinoma and pancreatic cancer, and may be a potential prognostic factor (8,9). hnRNP K coordinates with p53 to regulate its target gene transcription in response to DNA damage. Proteasome degradation of hnRNP K is mediated by E3 ligase MDM2 (10). The interaction between hnRNP K and c-Src leads to hnRNP K phosphorylation, which allows for hnRNP K activation of silenced mRNA translation (11).
• Synonyms: CSBP; HNRPK; TUNP

Reference Data

• Protein Pathways: Spliceosome