Lactate Dehydrogenase B (LDHB) Goat Polyclonal Antibody

CAT#: TA329084

LDHB Goat Polyclonal Antibody

Product Images

TA329084 (0.1 ug/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  TA329084 (0.1 ug/ml) staining of Pig Heart lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 0.03-0.1 µg/ml.
• Reactivities: Human, Mouse, Rat, Pig (Expected from sequence similarity: Dog, Cow)
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Internal region (near C terminus) (NARGLTSVINQKLK)
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: lot specific
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: lactate dehydrogenase B
• Database Link:
  NP_002291
  Entrez Gene 16832 MouseEntrez Gene 24534 RatEntrez Gene 477675 DogEntrez Gene 3945 Human
  UniProt ID P07195
• Background: Lactate dehydrogenase (LDH) is an ubiquitous enzyme commonly found in wide variety of organisms, including plants and microbes. LDH is involved in the interconversion of the pyruvate and NADH to lactate and NAD+. It is also called Hydroxybutyrate Dehydrogenase (HBD), because it can catalyze the oxidation of hydroxybutyrate (1). In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C. All LDH subunits can combine to form various terameric isoenzymes (140 kDa). Lactate dehydrogenase B (LDH-B, heart subunit, LDH-H) is involved in the conversion of L-lactate and NAD to pryruvate and NADH and it is predominantly localized in the heart tissue. Similar to other LDH subunit, LDH-B is considered to be an important marker for germ cell tumor (2).
• Synonyms: HEL-S-281; LDH-B; LDH-H; LDHBD; TRG-5

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Cysteine and methionine metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Propanoate metabolism, Pyruvate metabolism