H3FT (HIST3H3) Rabbit Polyclonal Antibody

CAT#: TA327574

DiMethyl-Histone H3-K14 Rabbit Polyclonal Antibody

Product Images

Western blot analysis of extracts of HeLa and H3 protein, using H3K14me2 antibody.

Specifications

Product Data

• Applications: ChIP, ChIP-seq, ICC/IF, IP, WB
• Recommended Dilution: WB 1:500 - 1:2000;IF 1:50 - 1:200;IP 1:50 - 1:200;ChIP 1:20 - 1:100
• Reactivities: Human, Mouse, Rat, Other (Wide Range)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic methylated peptide corresponding to residues surrounding K14 of human histone H3
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 15 kDa
• Gene Name: histone cluster 3, H3
• Database Link:
  NP_003484
  Entrez Gene 691496 RatEntrez Gene 8290 Human
  UniProt ID Q16695
• Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
• Synonyms: g; H3; H3.4; H3FT; H3t

Reference Data

• Protein Pathways: Systemic lupus erythematosus