IKK alpha (CHUK) Rabbit Polyclonal Antibody

CAT#: TA327250

Rabbit anti-CHUK Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using CHUK antibody.

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  Immunofluorescence analysis of A549 cell using CHUK antibody. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, IHC, IP, WB
• Recommended Dilution: WB 1:500 - 1:2000;IF 1:50- 1:200;IP 1:20- 1:100
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide of human CHUK
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: conserved helix-loop-helix ubiquitous kinase
• Database Link:
  NP_001269
  Entrez Gene 12675 MouseEntrez Gene 309361 RatEntrez Gene 1147 Human
  UniProt ID O15111
• Background: The NF-?B/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory I?B proteins. Most agents that activate NF-?B do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of I?B. The key regulatory step in this pathway involves activation of a high molecular weight I?B kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits.IKKa and IKKβ serve as the catalytic subunits of the kinase and IKK? serves as the regulatory subunit. Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKa), which causes conformational changes, resulting in kinase activation.
• Synonyms: IKBKA; IKK-alpha; IKK1; IKKA; NFKBIKA; TCF16

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Acute myeloid leukemia, Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Cytosolic DNA-sensing pathway, Epithelial cell signaling in Helicobacter pylori infection, MAPK signaling pathway, NOD-like receptor signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, RIG-I-like receptor signaling pathway, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway