PDHA1 Rabbit Polyclonal Antibody

CAT#: TA327210

Rabbit anti-PDHA1 Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using PDHA1 antibody.

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  Immunofluorescence analysis of U2OS cell using PDHA1 antibody. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, IHC, IP, WB
• Recommended Dilution: WB 1:500 - 1:2000;IF 1:50- 1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human PDHA1
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: pyruvate dehydrogenase (lipoamide) alpha 1
• Database Link:
  NP_000275
  Entrez Gene 18597 MouseEntrez Gene 29554 RatEntrez Gene 5160 Human
  UniProt ID P08559
• Background: The pyruvate dehydrogenase complex catalyzes the conversion of pyruvate and CoA into acetyl-CoA and CO2 in the presence of NAD+. Acetyl-CoA then goes into the citric acid cycle where it reacts with oxaloacetate to form citrate. Acetyl-CoA is also used for fatty acid and cholesterol biosynthesis. The reaction of oxidative decarboxylation of pyruvate therefore serves as a critical link between glycolysis and the citric acid cycle and lipid metabolism. In mammalian cells, the pyruvate dehydrogenase complex is located in the mitochondrial matrix . This complex is comprised of three enzymes: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and dihydrolipoamide dehydrogenase (E3). Pyruvate dehydrogenase (E1) consists of two subunits: a and β. This enzyme catalyzes the removal of CO2 from pyruvate. Mutations in the a subunits of pyruvate dehydrogenase (E1) lead to congenital defects that are usually associated with lactic acidosis, neurodegeneration and early death .
• Synonyms: PDHA; PDHAD; PDHCE1A; PHE1A

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Butanoate metabolism, Citrate cycle (TCA cycle), Glycolysis / Gluconeogenesis, Metabolic pathways, Pyruvate metabolism, Valine, leucine and isoleucine biosynthesis