Cyclin H (CCNH) Rabbit Polyclonal Antibody

CAT#: TA326887

CCNH Rabbit Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using CCNH antibody.

Specifications

Product Data

• Applications: IP, WB
• Recommended Dilution: WB 1:500 - 1:2000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human CCNH
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: cyclin H
• Database Link:
  NP_001230
  Entrez Gene 902 Human
  UniProt ID P51946
• Background: Cyclin H belongs to a conserved cyclin family that plays a critical role in the regulation of cell cycle dependent kinases (CDKs) necessary for cell cycle progression. In general, the activity of CDKs requires the binding of appropriate cyclins as well as phosphorylation driven by Cdk-activating kinase (CAK). Cyclin H is part of the CAK complex that includes the kinase CDK7, and an assembly factor p36/Mat1, which enhances binding between cyclin H and CDK7 and increases activity . CAK regulates progression through the cell cycle by activating cdc2, CDK2, and CDK4 kinases through phosphorylation of a critical threonine residue in the T-loop of the CDK-cyclin complexes . The CAK complex can exist either in its free form or in association with transcription factor IIH (TFIIH) which can affect its substrate specificity . When bound to TFIIH, CAK preferentially phosphorylates the carboxy-terminal domain of RNA polymerase II , providing a link between cell cycle control, transcriptional regulation, and DNA repair.
• Synonyms: CAK; CycH; p34; p37

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Cell cycle, Nucleotide excision repair