Perforin (PRF1) Rabbit Polyclonal Antibody

CAT#: TA326761

PRF1 Rabbit Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using Perforin antibody (TA326761) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

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  Immunohistochemistry analysis of paraffin-embedded mouse spleen using Perforin antibody (TA326761) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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  Immunohistochemistry analysis of paraffin-embedded rat lung using Perforin antibody (TA326761) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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  Immunofluorescence analysis of NIH/3T3 cells using Perforin antibody (TA326761) at dilution of 1:100. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, IF, IHC, WB
• Recommended Dilution: WB 1:500 - 1:1000
  IHC-P 1:50 - 1:200
  IF/ICC 1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human PRF1
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: perforin 1
• Database Link:
  NP_005032
  Entrez Gene 18646 MouseEntrez Gene 50669 RatEntrez Gene 5551 Human
  UniProt ID P14222
• Background: Granzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells. Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein. Granzyme B has the strongest apoptotic activity of all the granzymes as a result of its caspase-like ability to cleave substrates at aspartic acid residues thereby activating procaspases directly and cleaving downstream caspase substrates.Perforin is a pore-forming protein that facilitates the entry of cytotoxic serine proteases, such as granzymes, into target cells. Perforin is primarily expressed in cytotoxic T lymphocytes and NK cells.
• Synonyms: FLH2; HPLH2; P1; PFN1; PFP

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Allograft rejection, Autoimmune thyroid disease, Graft-versus-host disease, Natural killer cell mediated cytotoxicity, Type I diabetes mellitus, Viral myocarditis