HSP27 (HSPB1) Mouse Monoclonal Antibody [Clone ID: 5D12-A12]

CAT#: TA326365

Mouse monoclonal Hsp27 Antibody

Product Images

Western blot analysis of Hsp27 in a variety of cells lines using a 1:1000 dilution of the antibody

Specifications

Product Data

• Clone Name: 5D12-A12
• Applications: WB
• Recommended Dilution: WB: 0.25-0.5 ug/ml
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b, kappa
• Clonality: Monoclonal
• Immunogen: Human Hsp27
• Formulation: PBS pH7.4, 50% glycerol
• Concentration: lot specific
• Purification: Protein G Purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: heat shock protein family B (small) member 1
• Database Link:
  NP_001531
  Entrez Gene 3315 Human
  UniProt ID P04792
• Background: Hsp27s belong to an abundant and ubiquitous family of small heat shock proteins (sHSP). It is an important HSP found in both normal human cells and cancer cells. The basic structure of most sHsps is a homologous and highly conserved amino acid sequence, with an -crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers . Hsp27-oligomers consist of stable dimers formed by as many as 8-40 Hsp27 protein monomers . The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity .HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress,where it may function to stabilize DNA and/or the nuclear membrane. Other functions include chaperone activity (as mentioned above), thermotolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. Hsp27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that hsp27 may serve some role in cross-bridge formation between actin and myosin . And finally, Hsp27 is also thought to be involved in the process of cell differentiation. The up-regulation of Hsp27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that Hsp27 may play a crucial role in termination of growth .
• Synonyms: CMT2F; HEL-S-102; HMN2B; HS.76067; Hsp25; HSP27; HSP28; SRP27
• Note: Detects ~27kDa proteins corresponding to Hsp27 on SDS Page Immunoblots. Has no cross-reactivity to alphaB crystallin.

Reference Data

• Protein Pathways: MAPK signaling pathway, VEGF signaling pathway