PI 3 Kinase Class 3 (PIK3C3) Rabbit Polyclonal Antibody

CAT#: TA324651

Rabbit polyclonal PI3KC3 Antibody (S34)

Product Images

Western blot analysis of PI3KC3 (S34) (Cat. #TA324651) in Hela cell line lysates (35ug/lane). PI3KC3 (arrow) was detected using the purified Pab.

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  Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PI3KC3 Antibody (S34), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  IF image of U251 cells stained with PI3KC3 (S34) antibody. U251 cells were treated with Chloroquine, then incubated with TA324651 PI3KC3 (S34) primary antibody (1:200, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue). PI3KC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: IF: 1:200, WB: 1:1000, IHC: 1:10~50
• Reactivities: Human (Predicted: Mouse, Rat, Pig, Xenopus)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This PI3KC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-39 amino acids from human PI3KC3.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 101549 Da
• Gene Name: phosphatidylinositol 3-kinase catalytic subunit type 3
• Database Link:
  NP_002638
  Entrez Gene 65052 RatEntrez Gene 225326 MouseEntrez Gene 5289 Human
  UniProt ID Q8NEB9
• Synonyms: hVps34; VPS34; Vps34

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system, Regulation of autophagy