p38 (MAPK14) Rabbit Polyclonal Antibody

CAT#: TA324492

Rabbit polyclonal MAPK14 Antibody (Center T180/Y182)

Product Images

MAPK14 Antibody (Center T180/Y182) (Cat. #TA324492) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the MAPK14 antibody detected the MAPK14 protein (arrow).

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  MAPK14 Antibody (Center T180/Y182) (Cat. #TA324492)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MAPK14 Antibody (Center T180/Y182) for immunohistochemistry. Clinical relevance has not been evaluated.

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  IF image of A549 cell stained with MAPK14 Antibody (Center T180/Y182) (Cat#TA324492).A549 cells were incubated with MAPK14 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue).MAPK14 immunoreactivity is localized to Vesicles significantly and Cytoplasm weakly.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: IF: 1:10~50, WB: 1:1000, IHC: 1:10~50
• Reactivities: Human (Predicted: Mouse, Rat, Zebrafish)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This MAPK14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 158-192 amino acids from the Central region of human MAPK14.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 41293 Da
• Gene Name: mitogen-activated protein kinase 14
• Database Link:
  NP_001306
  Entrez Gene 26416 MouseEntrez Gene 81649 RatEntrez Gene 1432 Human
  UniProt ID Q16539
• Synonyms: CSBP; CSBP1; CSBP2; CSPB1; EXIP; Mxi2; p38; p38ALPHA; PRKM14; PRKM15; RK; SAPK2A

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Epithelial cell signaling in Helicobacter pylori infection, Fc epsilon RI signaling pathway, GnRH signaling pathway, Leukocyte transendothelial migration, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Progesterone-mediated oocyte maturation, RIG-I-like receptor signaling pathway, T cell receptor signaling pathway, Toll-like receptor signaling pathway, VEGF signaling pathway