Lin28 (LIN28A) Rabbit Polyclonal Antibody

CAT#: TA324388

Rabbit polyclonal LIN28A Antibody (Center)

Product Images

LIN28A Antibody (Center) (Cat. #TA324388) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the LIN28A antibody detected the LIN28A protein (arrow).

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  LIN28A Antibody (Center) (Cat. #TA324388)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of LIN28A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

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  IF image of SY5Y cells stained with TA324388 LIN28A (Center) antibody. SY5Y cells were incubated with TA324388 LIN28A (Center) primary antibody (1:200, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin. Lin28a immunoreactivity is localized very specifically to the nuclei of the SY5Y cells.

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  Immunofluorescent analysis of A549 cells, using LIN28A Antibody (Center) (Cat. #TA324388). TA324388 was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor® 554 (red) conjugated Phalloidin (red).

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  LIN28A Antibody (Center) (Cat. #TA324388) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: IF: 1:10~50, WB: 1:1000, IHC: 1:50~100, FC: 1:10~50
• Reactivities: Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This LIN28A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-138 amino acids from the Central region of human LIN28A.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 22743 Da
• Gene Name: lin-28 homolog A
• Database Link:
  NP_078950
  Entrez Gene 83557 Mouse
  UniProt ID Q9H9Z2
• Synonyms: CSDD1; LIN-28; lin-28A; LIN28; ZCCHC1

Reference Data

• Protein Families: Transcription Factors