MET Mouse Monoclonal Antibody [Clone ID: 4AT44]

CAT#: TA324318

Mouse monoclonal MET/HGFR Antibody

Product Images

Detection of endogenous Met in HepG2 cell line. 10 ug/lane of HepG2 cell lysate was used to examine the expression of human Met. Lanes 1-5 represent Abgent Ã?s different anti-Met monoclonal antibodies that are Cat# AM1001-1005. Lane 6 represents auto-phosohorylated-Met in HepG2 cell line detected by anti-phospho-Met Mab (Cat# AM1000).

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  Western blot analysis of lysates from Hela, HepG2, COS-7 cell line (from left to right), using MET/HGFR Antibody (4AT44). 4AT44 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

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  Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with MET/HGFR Antibody (Cat.#TA324318), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  IF image of HepG2 cells stained with MET/HGFR antibody. HepG2 cells were incubated with TA324318 MET/HGFR primary antibody (1:100, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue). Note the highly specific localization of the MET immunosignal to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000105976).

Specifications

Product Data

• Clone Name: 4AT44
• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:100~500, IF: 1:100, IHC: 1:50~100
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: This monoclonal antibody is generated from mice immunized with purified recombinant protein encoding the catalytic domain of human Met.
• Formulation: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 155541 Da
• Gene Name: MET proto-oncogene, receptor tyrosine kinase
• Database Link:
  NP_000236
  Entrez Gene 17295 MouseEntrez Gene 4233 Human
  UniProt ID P08581
• Synonyms: AUTS9; c-Met; DFNB97; HGFR; RCCP2

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Adherens junction, Axon guidance, Colorectal cancer, Cytokine-cytokine receptor interaction, Endocytosis, Epithelial cell signaling in Helicobacter pylori infection, Focal adhesion, Melanoma, Pathways in cancer, Renal cell carcinoma