GSK3 beta (GSK3B) Rabbit Polyclonal Antibody

CAT#: TA323991

Anti-GSK3B (Phospho-Tyr216) Rabbit Polyclonal Antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane 1-2: A549 and Hela cell lysates Primary antibody: TA323991 (GSK3B (Phospho-Tyr216) Antibody) at dilution 1/800 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 3 minutes

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  Immunohistochemistry of paraffin-embedded Human tonsil tissue using TA323991 (GSK3B (Phospho-Tyr216) Antibody) at dilution 1/20 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human tonsil tissue using TA323991 (GSK3B (Phospho-Tyr216) Antibody) at dilution 1/20, treated with synthetic peptide. (Original magnification: ×200)

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: A549 and Hela cell lysates
  IHC: 20-100
  Positive control: Human tonsil
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of tyrosine 216 (V-S-Y(p)-I-C) derived from Human GSK3β.
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 47 kDa
• Gene Name: glycogen synthase kinase 3 beta
• Database Link:
  NP_002084
  Entrez Gene 56637 MouseEntrez Gene 84027 RatEntrez Gene 2932 Human
  UniProt ID P49841
• Background: Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin.
• Synonyms: glycogen synthase kinase-3 beta; glycogen synthase kinase 3 beta; GSK3beta isoform

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Alzheimer's disease, Axon guidance, Basal cell carcinoma, B cell receptor signaling pathway, Cell cycle, Chemokine signaling pathway, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Hedgehog signaling pathway, Insulin signaling pathway, Melanogenesis, Neurotrophin signaling pathway, Pathways in cancer, Prostate cancer, T cell receptor signaling pathway, Wnt signaling pathway