Aminoacylase 1 (ACY1) Rabbit Polyclonal Antibody

CAT#: TA323595

Anti-ACY1 rabbit polyclonal antibody

Product Images

Gel: 10%SDS-PAGE Lysate: 40 μg Lane 1-3: Mouse kidney tissue human normal kidney tissue K562 cells Primary antibody: TA323595 (ACY1 Antibody) at dilution 1/200 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 minute

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  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA323595 (ACY1 Antibody) at dilution 1/20 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA323595 (ACY1 Antibody) at dilution 1/20, treated with fusion protein. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 200-1000
  WB positive control: Mouse kidney and human normal kidney tissue, K562 cells
  IHC: 25-100
  Positive control: Human thyroid cancer
  Predicted cell location: Cytoplasm and Nucleus
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein corresponding to C terminal 250 amino acids of human aminoacylase 1
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46 kDa
• Gene Name: aminoacylase 1
• Database Link:
  NP_000657
  Entrez Gene 109652 MouseEntrez Gene 95 Human
  UniProt ID Q03154
• Background: This gene encodes a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and an acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. This gene is located on chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and this enzyme is the first member of a new family of zinc-binding enzymes. Mutations in this gene cause aminoacylase-1 deficiency, a metabolic disorder characterized by central nervous system defects and increased urinary excretion of N-acetylated amino acids. Alternative splicing of this gene results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ABHD14A (abhydrolase domain containing 14A) gene, as represented in GeneID:100526760. A related pseudogene has been identified on chromosome 18.
• Synonyms: ACY-1; ACY1D; HEL-S-5

Reference Data

• Protein Families: Protease
• Protein Pathways: Arginine and proline metabolism, Metabolic pathways