Chk2 (CHEK2) Rabbit Polyclonal Antibody

CAT#: TA323481

Anti-CHEK2 Rabbit Polyclonal Antibody

Product Images

Gel: 6%SDS-PAGE Lysate: 40 μg Lane 1-5: MCF7 cells 293T cells HT29 cells Hela cells Jurkat cells Primary antibody: TA323481 (CHEK2 Antibody) at dilution 1/150 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 2 minutes

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 200-1000
  WB positive control: MCF-7, 293T, HT-29, Hela and Jurkat cells
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a region derived from 2-18 amino acids of Human Checkpoint kinase 2
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 61 kDa
• Gene Name: checkpoint kinase 2
• Database Link:
  NP_001005735
  Entrez Gene 11200 Human
  UniProt ID O96017
• Background: In response to DNA damage and replication blocks; cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated; the encoded protein is known to inhibit CDC25C phosphatase; preventing entry into mitosis; and has been shown to stabilize the tumor suppressor protein p53; leading to cell cycle arrest in G1. In addition; this protein interacts with and phosphorylates BRCA1; allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome; a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also; mutations in this gene are thought to confer a predisposition to sarcomas; breast cancer; and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene.
• Synonyms: CDS1; CHK2; hCds1; HuCds1; LFS2; PP1425; RAD53

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Stem cell - Pluripotency
• Protein Pathways: Cell cycle, p53 signaling pathway