Topoisomerase II alpha (TOP2A) Rabbit Polyclonal Antibody

CAT#: TA323277

Anti-TOP2A Rabbit Polyclonal Antibody

Product Images

Immunohistochemistry of paraffin-embedded Human colon cancer tissue using TA323277 (TOP2A Antibody) at dilution 1/30 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human colon cancer tissue using TA323277 (TOP2A Antibody) at dilution 1/30, treated with synthetic peptide. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using TA323277 (TOP2A Antibody) at dilution 1/30 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using TA323277 (TOP2A Antibody) at dilution 1/30, treated with synthetic peptide. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC
• Recommended Dilution: IHC: 50-200
  Positive control: Human colon cancer
  Predicted cell location: Cytoplasm, Nucleus
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a region derived from 1517-1531 amino acids of human topoisomerase (DNA) II alpha
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: topoisomerase (DNA) II alpha
• Database Link:
  NP_001058
  Entrez Gene 21973 MouseEntrez Gene 360243 RatEntrez Gene 7153 Human
  UniProt ID P11388
• Background: This gene encodes a DNA topoisomerase; an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation; chromatid separation; and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another; thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form; alpha; is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.
• Synonyms: TOP2; TP2A

Reference Data

• Protein Families: Druggable Genome