CKMT1B Rabbit Polyclonal Antibody

CAT#: TA322243

Anti-CKMT1A/CKMT1B Rabbit Polyclonal Antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane: MCF7 cell lysate Primary antibody: TA322243 (CKMT1A/CKMT1B Antibody) at dilution 1/600 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 15 seconds

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  Immunohistochemistry of paraffin-embedded Human gastric cancer tissue using TA322243 (CKMT1A/CKMT1B Antibody) at dilution 1/75 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human gastric cancer tissue using TA322243 (CKMT1A/CKMT1B Antibody) at dilution 1/75, treated with synthetic peptide. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA322243 (CKMT1A/CKMT1B Antibody) at dilution 1/75 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA322243 (CKMT1A/CKMT1B Antibody) at dilution 1/75, treated with synthetic peptide. (Original magnification: ×200)

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: MCF7 cell lysate
  IHC: 50-300
  Positive control: Human gastric cancer
  Predicted cell location: Cytoplasm and Nucleus
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide peptide corresponding to a region derived from 32-46 amino acids of human creatine kinase, mitochondrial 1B
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: creatine kinase, mitochondrial 1B
• Database Link:
  NP_066270
  Entrez Gene 1159 Human
  UniProt ID P12532
• Background: Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier; creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes; sarcomeric MtCK and ubiquitous MtCK; encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers; in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins.
• Synonyms: CKMT; CKMT1; UMTCK

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Arginine and proline metabolism, Metabolic pathways