ADO Rabbit Polyclonal Antibody

CAT#: TA322128

Anti-ADO Rabbit Polyclonal Antibody

Product Images

Gel: 10%SDS-PAGE Lysate: 30 μg Lane: Mouse testis tissue Primary antibody: TA322128 (ADO Antibody) at dilution 1/1200 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 30 minutes

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  Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TA322128 (ADO Antibody) at dilution 1/30 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TA322128 (ADO Antibody) at dilution 1/30, treated with synthetic peptide. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1000-5000
  WB positive control: Mouse testis tissue
  IHC: 25-100
  Positive control: Human liver cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a region derived from 161-174 amino acids of Human 2-aminoethanethiol (cysteamine) dioxygenase
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 30 kDa
• Gene Name: 2-aminoethanethiol (cysteamine) dioxygenase
• Database Link:
  NP_116193
  Entrez Gene 211488 MouseEntrez Gene 84890 Human
  UniProt ID Q96SZ5
• Background: Human thiol dioxygenases include cysteine dioxygenase (CDO; MIM 603943) and cysteamine (2-aminoethanethiol) dioxygenase (ADO; EC 1.13.11.19). CDO adds 2 oxygen atoms to free cysteine; whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine. Mouse Ado has strong and specific dioxygenase activity in vitro towards cysteamine but not cysteine. Recombinant Ado was shown to bind iron. Overexpression of Ado in HepG2/C3A cells increased the production of hypotaurine from cysteamine. Similar results were found with human ADO. When endogenous expression of ADO was reduced by RNA-mediated interference; hypotaurine production decreased. The demonstration of high levels of ADO in brain challenges the previous assumption that most of the taurine in the brain is a consequence of CDO activity.
• Synonyms: C10orf22

Reference Data

• Protein Pathways: Metabolic pathways, Taurine and hypotaurine metabolism