RAD50 Rabbit Polyclonal Antibody

CAT#: TA321722

Anti-RAD50 Rabbit Polyclonal Antibody

Product Images

Predicted band size: 154 kDa. Positive control: 293T and Hela cell lysate. Recommended dilution: 1/1000-5000. (Gel: 8%SDS-PAGE Lane 1: 293T cell lysate Lane 2: Hela cell lysate Lysates: 40 ug per lane Primary antibody: 1/1500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 minutes)

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:2000-10000, WB: 1:1000-5000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a region derived from 325-339 amino acids of Human RAD50 homolog (S. cerevisiae)
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 154 kDa
• Gene Name: RAD50 double strand break repair protein
• Database Link:
  NP_005723
  Entrez Gene 19360 MouseEntrez Gene 64012 RatEntrez Gene 10111 Human
  UniProt ID Q92878
• Background: The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50; a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein; cooperating with its partners; is important for DNA double-strand break repair; cell cycle checkpoint activation; telomere maintenance; and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.
• Synonyms: hRad50; NBSLD; RAD502

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Homologous recombination, Non-homologous end-joining