Cytokeratin 6C (KRT6C) Mouse Monoclonal Antibody [Clone ID: C11]

CAT#: TA319603

Mouse monoclonal Keratin antibody

Product Images

WB using Immunochemical's Mouse Anti-Keratin antibody. This antibody recognizes a single 56 kDa band corresponding to human keratin as confirmed by the position of molecular weight markers (not shown). A 1:400 dilution of Mab anti-Keratin was used for 2h followed by detection using a 1:5,000 dilution of IRDyeTM800 conjugated Goat-a-Mouse IgG [H&L] and visualization using the Odyssey® Infrared Imaging System developed by LI-COR.

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  IF of Immunochemical's Anti-Keratin antibody (TA319603) was used with Dylight 488 goat anti-mouse (shown in green) to detect Keratin by IF. In the same experiment, polyclonal Anti-HDAC-1 antibody (TA319251) was used with Atto425 Anti-Rabbit IgG (shown in red) to detect HDAC-1.

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  Immunofluorescence using Immunochemical's Mouse Anti-Keratin antibody. Confocal slices of HeLa cells are between 0.5 and 0.6 µm where the image is taken near the bottom of the cell. Use FITC a 1:2,000 dilution of FITC conjugated Goat-a-Mouse IgG [H&L] (610-102-121) for detection

Specifications

Product Data

• Clone Name: C11
• Applications: IF, WB
• Recommended Dilution: ELISA: 1:5,000 - 1:20,000, WB: 1:50 - 1:200, IHC: 1:50 - 1:200, IF: 1:50 - 1:200, IP: 1:100
• Reactivities: Human
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with purified human cytoskeletal preparations from A431 cells.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: keratin 6C
• Database Link:
  NP_775109
  Entrez Gene 286887 Human
  UniProt ID P48668
• Synonyms: K6E; KRT6E; PPKNEFD
• Note: Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (i.e. skin) and non-keratinizing cells (i.e. mesothelial