GGA3 Rabbit Polyclonal Antibody

CAT#: TA319379

Rabbit polyclonal anti-GGA3 antibody

Product Images

WB using Anti-GGA3 antibody shows detection of a band at ~110 kDa corresponding to GFP-GGA3 fusion protein present in a lysate of HEK293 cells over- expressing the recombinant protein (lane 2, arrowhead). Pre-incubation of antibody with immunizing peptide blocks specific staining (lane 3). MW markers are shown in lane 1 (700 nm channel - red).The primary antibody was diluted to 1:600.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:10,000 - 1:50,000, WB: 1:500 - 1:3,000, IP: 1:100
• Reactivities: Human, Chimpanzee
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 400-415 of Human GGA3.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: golgi associated, gamma adaptin ear containing, ARF binding protein 3
• Database Link:
  NP_619525
  Entrez Gene 23163 Human
  UniProt ID Q9NZ52
• Synonyms: KIAA0154
• Note: GGA3 (also known as ADP-ribosylation factor binding protein GGA3, Golgi-localized, gamma ear-containing, ARF-binding protein 3, Gamma-adaptin related protein 3) plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. This protein mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. GGA1 is a monomeric protein that interacts with SORT1, SORL1, LRP3, GGA binding partner (GGABP) and P200, GGA1 and GGA2 as well as RABEP1, RABGEF1 and type-I membrane proteins M6PR/CD-MPR, IGF2R/CI-MPR and BACE1. GGA3 is a ubiquitously expressed membrane-associated protein that is localized to TGN and endosomes. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Lysosome