MET Rabbit Polyclonal Antibody

CAT#: TA319135

Rabbit polyclonal c-Met phospho Y1349 / Y1356 antibody

Product Images

Western blot using affinity purified anti-c-Met pY1349pY1356 antibody shows detection of phosphorylated c-Met. Human mammary B5/589 epithelial cells were serum-deprived and treated with or without HGF. Cell lysates were immunoprecipated with the anti-c-Met antibody, resolved by SDS-PAGE, transferred to PVDF membrane, and probed with anti-c-Met pY1349pY1356. Personal communication, D. Bottaro and T. Ito, NCI, Bethesda, MD

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  WB using Anti-c-Met pY1349pY1356 shows detection of phosphorylated c-Met. Human mammary epithelial cells (B5/589) were serum deprived and stimulated with (+) and without (-) HGF. Cell lysates were IP'd using human anti-c-Met, and probed using various antibodies. Lane 1 and 2 where probed using the anti-c-Met pY1349pY1356, lane 3 and 4 where probed using an anti-phosphotyrosine as phosphorylation control and lane 5 and 6 where probed using an anti-cMet as a total Met loading control. Bands recognized at MW of ~170 kDa are total Met, ~145 kDa phosphorylated Met beta chain in HGF +, ~150 kDa phosphorylated Met in +/- HGF, ~50kDa phosphorylated Met alpha chain.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:93,000, WB: 1ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y1349 and Y1356 of human c-Met protein.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: MET proto-oncogene, receptor tyrosine kinase
• Database Link:
  NP_000236
  Entrez Gene 17295 MouseEntrez Gene 24553 RatEntrez Gene 4233 Human
  UniProt ID P08581
• Synonyms: AUTS9; c-Met; DFNB97; HGFR; RCCP2
• Note: This antibody is designed, produced, and validated for Cancer, Immunology and Nuclear Signaling research. Anti-c-MET is the receptor for hepatocyte growth factor (also known as scatter factor, HGF/SF), and belongs to the tyrosine kinase superfamily. Interaction of c-Met with HGF results in autophosphorylation of c-Met at multiple tyrosines. Phosphorylation of Y1234/1235 in the c-Met kinase domain is critical to kinase activation. When phosphorylated, Y1349 and Y1356, along with surrounding amino acids, form a unique bidentate docking site for substrates such as Gab1, Grb2, phosphatidylinositol 3-kinase (PI3K) and others. C-Met mainly uses the Gab1 scaffolding adaptor in its initial step of signal transmission. Well-characterized downstream signalling pathways that are activated by c-Met include the ERK/MAPK, PI3K–Akt/PKB, Crk–Rap and Rac–Pak pathways, resulting in proliferation and increased cell survival.

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Adherens junction, Axon guidance, Colorectal cancer, Cytokine-cytokine receptor interaction, Endocytosis, Epithelial cell signaling in Helicobacter pylori infection, Focal adhesion, Melanoma, Pathways in cancer, Renal cell carcinoma